FastQCFastQC Report
Mon 6 Jun 2016
ERR1391268.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391268.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662790
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA41290.6229725855851778No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA28000.42245658504201933No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTGAGATCTCGTATGCCGTC15320.23114410295870486Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTGAGATCTCGTATGCC10170.15344226678133344Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT8810.13292294693643536Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTCG1000.045.00920510
GCCGATG4450.042.9733516
CGGTATT4200.042.85297424
GCGGTCT4150.042.8271636
GATTCGG1000.042.75874311
CGATGAC4500.042.49586518
CAGGCGG4200.042.31731433
TCGGTAT4200.042.31731423
AGCCGAT4550.042.0288815
GACGTCA4550.042.0288822
GAATTCG4250.041.8194619
CCGATGA4600.041.5720417
CGTCATA4550.041.53442424
TCGGGGA4300.041.3331914
AATTCGG4300.041.3331920
TTCGGTA4300.041.3331922
GGCGGTC4250.041.29010435
GATGACG4650.041.12503419
ATTCGGT4350.040.85809721
CGGGGAA4350.040.85809715