ERR1391259.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1391259.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	660550
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA	4088	0.6188782075543108	No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA	2706	0.4096586178184846	No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACACATCTCGTATGCCGTC	2281	0.3453182953599273	Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACACATCTCGTATGCC	1831	0.2771932480508667	Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT	1018	0.15411399591249716	Illumina Paired End PCR Primer 2 (96% over 30bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TAAGCCG	435	44.995308	13
TATGCCG	280	43.388332	43
AGCCGAT	455	43.017487	15
GCCGATG	455	43.017487	16
ACGTCAT	455	43.017487	23
GACGTCA	460	42.549908	22
ATGACGT	460	42.549908	20
GATGACG	460	42.549908	19
CGTCATA	460	42.549908	24
AAGCCGA	465	42.09238	14
CCGATGA	470	41.644592	17
CGATGAC	470	41.644592	18
CGGGGAA	360	41.245697	15
TGACGTC	475	41.20623	21
ATGCCGT	295	41.182148	44
CGGTATT	365	40.68069	24
TCGGTAT	365	40.68069	23
GGCGGTC	360	40.62076	35
TTCGGTA	370	40.13095	22
GCGGTCT	365	40.064316	36