FastQCFastQC Report
Mon 6 Jun 2016
ERR1391257.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391257.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440082
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA46461.0557123445176126No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA32880.7471334887589132No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTCTAATCTCGTATGCCGTC9390.21336932662549252Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTCTAATCTCGTATGCC7940.18042092155552827Illumina Paired End PCR Primer 2 (96% over 33bp)
TCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA6450.14656359496639262No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT6040.1372471493948855Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG4400.044.48144516
AGCCGAT4450.043.98165515
CCGATGA4450.043.98165517
TGACGTC4500.043.4929721
TAAGCCG4500.043.4929713
ACGTCAT4550.043.01502223
CGATGAC4550.043.01502218
GACGTCA4600.042.54747422
AAGCCGA4600.042.54747414
TATGCCG800.042.18068343
ATGACGT4650.042.08997320
CGTCATA4650.042.08997324
AGATCGG1250.041.3933125
GATGACG4750.041.20386519
CGGTATT4650.041.12238724
GACCCTT4850.040.82278439
GCGGTCT4650.040.6432136
GGCGGTC4700.040.21083535
TTCGGTA4700.040.2062722
TCCTCGG3650.040.08934811