FastQCFastQC Report
Mon 6 Jun 2016
ERR1391256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612785
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA38450.6274631396003493No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA26740.43636838369085407No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTATCTGATCTCGTATGCCGTC9590.15649860881059424Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTATCTGATCTCGTATGCC7100.11586445490669647Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG4000.043.3065316
TATGCCG950.042.625743
GATGACG4100.042.25027519
CGTCATA4100.042.25027524
AAGCCGA4200.041.77995314
ACGTCAT4200.041.77995323
AGCCGAT4150.041.74123415
GACGTCA4150.041.74123422
ATGACGT4150.041.74123420
CGATGAC4150.041.74123418
TAAGCCG4150.041.74123413
TCGGTAT4000.041.61926323
AATTCGG4050.041.10544620
TTCGGTA4050.041.10544622
TGACGTC4250.040.7590921
GGCGGTC4050.040.5499735
CCGATGA4300.040.28514517
CGGTATT4150.040.11495224
GCGGTCT4150.039.5728636
AGATTCG800.039.3952910
Produced by FastQC (version 0.11.5)