FastQCFastQC Report
Mon 6 Jun 2016
ERR1391255.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391255.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652438
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA38870.595765421388699No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA30190.4627259601678627No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATACTCATCTCGTATGCCGTC12580.1928152560090001Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACTCATCTCGTATGCC7230.1108151272611344Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT4600.044.01828815
CGTCATA4700.043.08173424
GACGTCA4750.042.62824222
TGACGTC4750.042.62824221
GCCGATG4700.042.60304616
TAAGCCG4700.042.60304613
ACGTCAT4850.041.74930623
GCGGTCT4000.041.6217436
GGCGGTC4000.041.6217435
TCGGTAT4000.041.6217423
AAGCCGA4900.041.3232914
TTCCTCG3600.041.2499310
TTCGGTA4050.041.1078922
ATGACGT4950.040.90588420
GATGACG4950.040.90588419
TCGGGGA4150.040.65946614
CGGGGAA4150.040.65946615
CGGTCTC4150.040.65946637
ATTCGGT4100.040.60657521
CGATGAC5000.040.49682618
Produced by FastQC (version 0.11.5)