FastQCFastQC Report
Mon 6 Jun 2016
ERR1391254.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391254.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452404
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA41080.9080379483824192No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA31190.6894280333507219No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCACTAATCTCGTATGCCGTC9200.20335806049460217Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCACTAATCTCGTATGCC7090.15671833140290536Illumina Paired End PCR Primer 2 (96% over 33bp)
TCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA5880.12997232562046312No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT5500.12157275355655565Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCA4450.043.4806922
CGTCATA4350.043.44582424
GCCGATG4400.042.9521216
ATGACGT4400.042.9521220
ACGTCAT4400.042.9521223
TAAGCCG4400.042.9521213
TGACGTC4500.042.497621
AGCCGAT4450.042.4695115
CCGATGA4450.042.4695117
CGATGAC4450.042.4695118
GATGACG4500.041.99762719
AAGCCGA4550.041.53611414
TATGCCG950.040.26088343
TTCGGTA3700.039.52479622
TCGGTAT3750.038.997823
CGGGGAA3800.038.4846715
GAATTCG3800.038.4846719
AGGCGGT3850.037.98486734
GGCGGTC3850.037.98486735
ATTCGGT3950.037.5928121