FastQCFastQC Report
Mon 6 Jun 2016
ERR1391249.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391249.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692749
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA42830.618261448230167No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA32000.4619277689321818No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTAGTATCTCGTATGCCGTC11690.16874798808803768Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTAGTATCTCGTATGCC10500.15157004918087214Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACGT5700.043.02148420
CGTCATA5700.042.62679324
TTCGGTA3700.042.56278622
TAAGCCG5800.042.27973613
TGACGTC5750.042.25612321
ACGTCAT5750.042.25612323
TCGGTAT3750.041.99528523
GATGACG5900.041.94444319
AGCCGAT5850.041.9183715
GACGTCA5800.041.89184622
GCCGATG5900.041.5631316
CGATGAC5900.041.5631318
GGCGGTC3900.041.533835
CGGTCTC3900.041.533837
CGGTATT3800.041.44271524
CCGATGA5950.041.21385617
TCGGGGA3900.040.9569414
GAATTCG3950.040.43849619
AATTCGG4000.039.93301420
AAGCCGA6150.039.87357314
Produced by FastQC (version 0.11.5)