FastQCFastQC Report
Mon 6 Jun 2016
ERR1391248.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391248.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670650
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA38550.5748154775218072No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA29380.43808245731752776No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATCTATCTCGTATGCCGTC15340.228733318422426Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATCTATCTCGTATGCC12250.18265861477670917Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT7220.10765675091329308Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCA4550.044.0052322
AGCCGAT4500.042.99437315
TAAGCCG4650.042.57507313
GCCGATG4550.042.52190816
ACGTCAT4700.042.12214723
TGACGTC4600.042.0597121
CGTCATA4750.041.67875324
ATGACGT4750.041.2051320
GATGACG4700.041.16482519
AAGCCGA4850.040.819414
CGATGAC4800.040.30722418
GGCGGTC3750.040.1947435
TATGCCG1750.039.85192543
TGCCGTC1700.039.7243845
CGGTATT3950.039.29865324
TTCGGTA3900.039.2256322
TCGGTAT3900.039.2256323
GAATTCG3950.038.72910719
CCGATGA5000.038.69493517
AGGCGGT3950.038.1595634
Produced by FastQC (version 0.11.5)