FastQCFastQC Report
Mon 6 Jun 2016
ERR1391247.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391247.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545305
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA32740.6003979424358845No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA23820.43681976141792206No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTATCAATCTCGTATGCC12030.22061048404104128Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTATCAATCTCGTATGCCGTC11650.21364190682278728Illumina Paired End PCR Primer 2 (96% over 32bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT5750.1054455763288435Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA3850.044.4090724
CCGATGA3750.044.3934917
AGCCGAT3850.043.8247415
GATGACG3800.043.8093619
ACGTCAT3900.043.26288223
GACGTCA3850.043.2404122
TGACGTC3850.043.2404121
ATGACGT3850.043.2404120
CGATGAC3850.043.2404118
TAAGCCG4000.042.18131313
GCCGATG4050.041.10508316
TGCCGTC1550.040.64665245
TATGCCG1550.040.639243
AAGCCGA4300.039.7616114
TTCGGTA2300.039.1246922
CGGTATT2300.039.1246924
TCGGTAT2300.039.1246923
ATGCCGT1700.038.3767244
GGCGGTC2350.038.2922535
TCGGGGA2450.037.64753714
Produced by FastQC (version 0.11.5)