FastQCFastQC Report
Mon 6 Jun 2016
ERR1391245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672531
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA50710.7540172869354721No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA35830.5327635454722532No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTACTATCTCGTATGCCGTC7690.11434417149544036Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTACTATCTCGTATGCC6770.10066450468454242Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCA4050.043.88105822
AGCCGAT4200.043.38511315
GCCGATG4200.043.38511316
TAAGCCG4200.043.38511313
TCGGTAT4400.042.43560823
CCGATGA4300.042.3761617
TGACGTC4200.042.31387721
ATGACGT4200.042.31387720
ACGTCAT4200.042.31387723
CGTCATA4200.042.31387724
AAGCCGA4400.041.92433514
CGATGAC4350.041.88907618
GCGGTCT4500.041.49259636
GGCGGTC4500.041.49259635
TTCGGTA4550.041.0366322
TGCCGTC1050.040.72821845
CGGTATT4650.040.6379124
AGGCGGT4600.040.5905834
CGGGGAA4600.040.5905815
GAATTCG4600.040.5905819
Produced by FastQC (version 0.11.5)