FastQCFastQC Report
Mon 6 Jun 2016
ERR1391242.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391242.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638511
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA35690.558956697691974No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA29700.4651446881886138No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTACACTATCTCGTATGCCGTC11180.17509486915652198Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACACTATCTCGTATGCC9630.15081964132176268Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT4500.044.49442715
GCCGATG4500.043.99448816
AAGCCGA4550.043.5110314
TAAGCCG4600.043.03808613
ACGTCAT4650.042.57530623
CGTCATA4650.042.57530624
TTCGGTA4250.042.34763322
TGACGTC4700.042.1223821
GATGACG4700.042.1223819
GCGGTCT4300.041.8552236
GACGTCA4750.041.6789922
ATGACGT4750.041.6789920
TATGCCG1350.041.66144643
TCCTCGG3350.041.65614711
TCGGTAT4350.041.37412623
CCGATGA4800.041.2448317
CGATGAC4800.041.2448318
GGCGGTC4400.040.90396535
GATTCGG1000.040.5139611
AATTCGG4450.040.4443720
Produced by FastQC (version 0.11.5)