FastQCFastQC Report
Mon 6 Jun 2016
ERR1391237.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391237.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542722
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA36220.667376667981029No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA27130.4998876035981589No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCACATATCTCGTATGCCGTC13260.24432398170702493Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCACATATCTCGTATGCC11680.21521147106621807Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT5870.10815850472249144Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCA207.0310535E-444.9986223
TCGGTAT3750.043.19867723
AGCCGAT3300.042.9492715
GCCGATG3300.042.9492716
ATGACGT3350.042.31213420
CGATGAC3350.042.31213418
TTCCTCG3300.042.2792210
TATGCCG1550.042.0954843
TTCGGTA3850.042.0766322
CGGTATT3850.042.0766324
TCGGGGA3850.042.07275414
GATGACG3400.041.68989619
CCGATGA3400.041.68605417
TAAGCCG3400.041.68605413
CGGGGAA3900.041.5333615
GACGTCA3450.041.08569722
TGACGTC3450.041.08569721
CGTCATA3450.041.08569724
GCGGTCT3950.041.00761836
CGGTCTC3950.041.00761837
Produced by FastQC (version 0.11.5)