FastQCFastQC Report
Mon 6 Jun 2016
ERR1391231.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391231.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757943
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCA300.005073785330.0870233
GACCGTT450.001229559224.98816329
CGTATGC500.002268402422.49231344
CGGTGAG550.003943113620.4475642
AGACCGT550.003946128320.4448628
CGTTTAG550.003946128320.4448632
CCGTTTA550.003946128320.4448631
CTCGTAT600.00652134818.74359342
TCGTATA800.001715546716.867019
ATCTTCG1104.5206012E-516.4111045
GAGCGGT850.00251499715.9284256
CGTATAA900.00376419114.99289810
CGCCACC1555.6490946E-513.1024135
AATCGCT1403.6183733E-412.85105313
CCGGGAG1454.8645184E-412.40791525
GGCCGAG2003.6715355E-612.37077243
GAATCGC1300.00303059812.10964812
AGGCCGA2301.3580866E-611.7351242
GTATGCC1350.00401516711.66268145
TCTTCGT1558.282796E-411.6465896
Produced by FastQC (version 0.11.5)