FastQCFastQC Report
Mon 6 Jun 2016
ERR1391228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633865
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA453.502371E-529.98243741
CGCCCGG702.8983777E-828.91163413
CCGTTTG507.178137E-526.98631931
CTCGTAT507.181992E-526.9841942
TCGTATG507.181992E-526.9841943
CGTATGC551.3727027E-424.53108444
CGTATAA551.3727027E-424.53108410
AAACGGA651.5283369E-524.2184922
AGACCGT602.4742863E-422.48860228
ATCTCGT602.475608E-422.48682840
CATCTCG754.619248E-520.98770539
GACCGTT654.2498513E-420.75870729
ACGCCCG550.00394798820.4425712
GAGACCG707.0025795E-419.27594427
CAAACGG600.006526412418.74050121
TCGTATA750.00111384517.9894629
CGGAGGT901.8712475E-417.49113533
GAGCGGT800.001670726816.9332436
CGTTTGA800.001715508516.86645132
TGCCGAG800.001715508516.86645123
Produced by FastQC (version 0.11.5)