FastQCFastQC Report
Mon 6 Jun 2016
ERR1391227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624618
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAA301.1430905E-437.47898529
ACCGAAA301.1430905E-437.47898530
ATCTCGT450.001229939724.9859940
CTCGTAT450.001229939724.9859942
GAGACCG500.00227084522.48739227
TCTCGTA500.00227084522.48739241
GCGCTCC500.00227084522.4873928
CGCTCCA654.2512975E-420.7575919
CGTATGC550.0039473520.44308344
AAAGCGC706.8146933E-419.3539245
AGCGCTC600.00652833618.7394927
AAGCGCT750.001083470318.063666
GTCCCTA800.001716087916.86554338
GTATGCC952.828838E-416.56965845
GATCTCG850.0025730115.87345339
GAGACGG1350.00402038411.66012941
GCTAATT4253.274181E-1110.58314919
GTGCCAC2005.9979927E-410.1608094
CTGGCTA2302.0586459E-49.77712716
CCGTCTC1900.00448097439.507193
Produced by FastQC (version 0.11.5)