FastQCFastQC Report
Mon 6 Jun 2016
ERR1391225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727330
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCCG250.002112470335.97959538
ATCGTGC550.003853161620.5290415
CGCCTGG707.006774E-419.27478213
GATCGTG600.006373055318.8182894
CGTGAGC1454.693088E-412.4607261
CCGAGTA1300.002938518512.159514
GACGGGG1853.0347356E-410.93974243
TCGTTGG1450.006638610710.901634
CGAGTAG1450.006638610710.901635
CGAACTC1450.006845786710.8566598
ACTAGAC2554.6993155E-610.58223419
GAAACGG1700.001844730610.58223310
GTGTATT1700.001844730610.58223326
GGAAACG1700.001844730610.5822339
CGTTGGA1500.00853856910.5382415
AAACGGG1750.002347540310.27988411
CTAGACA5101.8189894E-1210.14130820
CCACGCC2006.2310975E-410.11926210
TTCGTTG1800.002864010410.0364213
ACCACGC1800.00296553349.9943329
Produced by FastQC (version 0.11.5)