FastQCFastQC Report
Mon 6 Jun 2016
ERR1391224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871502
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGC250.002112930635.97879415
ATCTTCG1503.2923708E-1019.5648445
TCGTATA1403.105015E-919.275469
CTTCGTA1555.366019E-1018.8609357
ACTAGAC3700.018.8424119
TTCGTAT1507.3050614E-917.990438
CGTATAA1601.6223566E-816.86602810
ACCACGC1552.0768675E-715.9592529
CTCGTGA1602.9746116E-715.462342
TCTTCGT2051.2551027E-915.416956
AGTTCGA1209.4665214E-515.0516131
CGGCTAA1056.0618226E-414.99116417
TCGTGAT1801.1266475E-613.744266543
CCGGGCG1555.6225246E-513.1094691
ACCTCGT1555.829407E-513.05906940
GTTCGAG1250.002196277512.641172
TTCGAGA1250.002196277512.641173
CCTTCGT1454.7081654E-412.4565081
CACCACG2003.6811434E-612.3684218
TCTCGAT1300.003031242212.10963927
Produced by FastQC (version 0.11.5)