FastQCFastQC Report
Mon 6 Jun 2016
ERR1391223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724635
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG606.4646883E-933.72883243
GACCGTT606.4683263E-933.72650529
CGTATGC651.4097168E-831.13645444
CTCGTAT651.4106263E-831.13430842
CCCCGAA300.00503419930.1350731
TATCTCG755.671245E-826.98120539
TCTCGTA801.0590884E-725.29662341
TTCGTAT651.5309754E-524.2139028
TCGTATA651.5309754E-524.2139029
CTTCGTA751.7065122E-623.9832947
AGACCGT952.1283995E-823.6677228
ATCTCGT1003.6639904E-822.48588840
CGTATAA702.7157817E-522.48433710
CCTTCGT1055.8169462E-821.5250531
GATCGGA654.2561023E-420.75477418
GAGACCG1009.1060974E-720.23590327
ACCGTTT1009.1060974E-720.23590330
CGTTTCT1102.268689E-618.39627532
CTTCGTT1253.6520214E-718.0810432
GTATGCC1253.8508733E-717.98995245
Produced by FastQC (version 0.11.5)