FastQCFastQC Report
Mon 6 Jun 2016
ERR1391221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850744
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGTC506.9683236E-527.1057451
ACGTCTT807.339581E-519.7634373
AACGTCT706.802843E-419.3601042
GATCGGA707.008177E-419.27475718
ACCACGC2500.018.8881519
CGTCTTC1253.678615E-718.069434
ACGCCCA1002.0566837E-517.98871612
AGATCGG750.001114581517.98871417
CCACGCC2550.016.75419610
CGCCACC1501.3604404E-716.5636435
CACCACG2800.016.0622988
CACGCCC1451.8192186E-615.50751311
CGCCCGC1055.85817E-415.0587461
CCCGCCA1553.472942E-614.5721213
CCGAGAC1402.2418468E-514.45521845
GAAGCAT5200.014.27157227
CGCCTGG1758.2193037E-714.1339913
CCGCCAC1604.8261663E-614.1167414
ATCGGAA1000.007577324813.4923319
GCGGAGC1352.6634563E-413.32575832
Produced by FastQC (version 0.11.5)