FastQCFastQC Report
Mon 6 Jun 2016
ERR1391220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787502
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCGG908.2898005E-619.98769613
CGGAAAA600.00637306418.818521
GCCCGGC1502.5875015E-614.99077114
GGCGGAG1705.917609E-714.55079231
GCGGAGG1553.634399E-614.5081232
CGGCTAA1553.6367946E-614.50719917
CGTGAGC1604.8351776E-614.1138891
CCGGCTA1504.2919128E-513.49169416
CGGAGGT1555.835388E-513.05730833
GCCACCG1250.00219064812.6452386
AGTCTCG1300.002940027512.1588834
GTCTCGC1350.003899318611.7085545
AGGCGGA1953.8616046E-511.53209630
CCCGGCT2005.0027047E-511.24307915
AGTGAGC4303.6379788E-1210.9823143
GGAGGCG2056.428271E-510.96955428
CGGAGTC1450.00663883510.9017631
ACCACGC1500.0088130710.493549
ACTAGAC3257.244671E-810.38020419
GAATCGC1750.002348853710.279386512
Produced by FastQC (version 0.11.5)