FastQCFastQC Report
Mon 6 Jun 2016
ERR1391218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638377
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAC550.00394609220.44429228
TTGAACG550.00394609220.44429245
AGATCGA707.0024026E-419.27604543
CGGGTGT850.002508230715.9342991
ACCACGC1901.3368117E-714.2034039
GGAGGCG1000.007571250713.49323328
GTGTTGT1454.8637958E-412.40757119
CCCGTCT1300.00294599812.1550091
ACGCCCA1300.003030328812.1093112
GCCACCA5450.011.5974416
CACCACG2402.269193E-611.2443618
ACTAGAC2503.7027894E-610.79458519
CGTGAGC1500.00856010310.5343421
GTAACTT2201.3139173E-410.2221468
TGCCACC3552.284105E-810.1740135
CCACGCC2453.4935452E-510.09697610
CTAGACA3951.2198143E-89.6786920
TCAGTAA2657.924347E-59.370175
GATGGTC2250.00176707849.0294374
GAAGCAT3755.8070873E-78.99548827
Produced by FastQC (version 0.11.5)