FastQCFastQC Report
Mon 6 Jun 2016
ERR1391212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173098
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA453.5031633E-529.98459626
AGCGGAT300.005160242329.98459627
AGATCGG507.183589E-526.98613725
ATGCCGA709.346113E-725.70217919
ATCGGAA551.3730033E-424.53285227
TCGGAAG802.988132E-622.4884528
AACGGGT550.003949286420.44317212
AATGCCG951.3212224E-518.93844818
TGCCGAG1002.0550013E-517.99152620
AGAGCGG1053.0236819E-517.2007772
CGGAAGA952.8304808E-416.57043629
TCGTATA952.831401E-416.569739
GCGGTTC1104.5055927E-516.4189225
AGCGCTC1056.0625194E-414.991667
CCGGCTA1859.677751E-814.5864816
AGCGGTT1402.1601536E-514.5131564
TCGCCAG950.005395969414.20262516
GGGGGGC950.005395969414.20262537
GCGCTCC1150.001206859513.6880378
CGGTTCA1352.5773616E-413.3783836
Produced by FastQC (version 0.11.5)