FastQCFastQC Report
Mon 6 Jun 2016
ERR1391208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438770
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCTC300.00515020529.99247
ACGCCCG300.005155949429.98556512
CGCAGGA300.005155949429.98556536
GATCGGA406.173264E-428.11467418
CTCGACC450.00120529725.0708265
ATCGGAA450.001227972324.99081819
TGCACTC1602.9669536E-715.46130715
GCACTCC1602.9669536E-715.46130716
TCTCGAA1250.002200406712.6356965
ACTGCAC2201.0608284E-511.24458713
GCTCCAA1450.006682730710.8903511
ACCACAC1700.001839155710.58434733
CTGCACT2251.6456992E-49.99518914
TAGACAG2851.7550658E-59.47020421
TGGGACT2200.0014880069.20011613
CAGAAGC3807.004219E-78.87730525
CTAGACA2851.8164048E-48.68102120
AGTGAGC2350.0027099758.61287543
CACTCCA3401.4884254E-58.59880217
ACAGAAG4001.4621892E-68.434401524
Produced by FastQC (version 0.11.5)