FastQCFastQC Report
Mon 6 Jun 2016
ERR1391206.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391206.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954445
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGAT300.005159148529.9853333
ACGGTGT406.1830337E-428.11124624
TCTCGTA651.5291806E-524.2189241
CTCGTAT751.7043822E-623.98826442
TCGTATA1052.3810571E-923.5586689
CGTATAA1003.6636266E-822.4878210
ATCTTCG1257.221388E-1021.6700485
ACGCCCG955.5557757E-721.30536712
CCGAAGC754.618956E-520.9897331
CGCCCGG1401.382432E-1020.88264313
CGTATGC807.5897595E-519.67787244
GACCGAA807.5897595E-519.67787229
TCCCGAC1002.0546702E-517.99119813
CCGACCT901.8722477E-417.49144415
ACGAAGG1053.1260224E-517.1335759
ACTCCCG800.001716165216.86674711
CTTCGTA1358.6273394E-716.6576427
ATCTCGT952.82926E-416.5708440
CGAAGCT952.82926E-416.5708432
TCTTCGT1652.264278E-816.4167026
Produced by FastQC (version 0.11.5)