FastQCFastQC Report
Mon 6 Jun 2016
ERR1391204.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391204.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803614
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA551.3732474E-424.53082719
CTTCGTT1152.5102054E-1023.575762
GATCGGA500.00227187722.48659118
TCGGAAG500.00227187722.48659120
CCTTCGT1257.1304385E-1021.6896971
TTCGTTG1452.0736479E-1020.2561823
AAAGCGT600.00635493418.8278643
AACGGGT600.006533614418.73766112
TCGTTGG1607.712515E-1018.3571664
CGGAGTC1001.9737363E-518.0747491
CGTTGGA1701.7298589E-917.2773345
GGAAACG1951.1346856E-814.99012859
GCGAAAC900.003769083214.99012827
GAAACGG2052.1929736E-814.25890210
AAGAGCG950.00539544514.20205724
AAACGGG2103.0100637E-813.91940511
CTCGAAC2154.0990926E-813.59569711
AACGGGA1403.6244447E-412.848680512
CGGGATT1403.6244447E-412.848680514
TCGAACT2451.6285412E-812.848680512
Produced by FastQC (version 0.11.5)