FastQCFastQC Report
Mon 6 Jun 2016
ERR1391203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497366
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCCGG250.00211304235.97538833
GATCGGA501.9892177E-631.47846618
ATCGGAA501.9892177E-631.47846619
AGATCGG507.1837334E-526.98154317
CGTATGC507.1837334E-526.98154344
CGGGACT651.5295725E-524.21420532
CTCGTAT602.4762133E-422.48461742
TCGGAAG602.4762133E-422.48461720
TCTCGTA602.4762133E-422.48461741
TCGTATG602.4762133E-422.48461743
ACCGGGA654.2531564E-420.75503230
CCGGGAC707.0080213E-419.2725331
GACCGGG707.0080213E-419.2725329
CGCCACC600.00634384218.8318445
ACCACGC1053.124973E-517.131149
AGACCGG800.0017168416.86346428
CGAGACC952.8300448E-416.56761426
CACGCCC1104.656198E-516.3524511
ATCTCGT850.00257413915.87149640
CCACGCC1301.1110515E-515.56627410
Produced by FastQC (version 0.11.5)