FastQCFastQC Report
Mon 6 Jun 2016
ERR1391201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879836
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA1050.029.98196810
TCGTATA1100.028.619159
TTCGTAT1757.2759576E-1219.2752158
ATCTTCG1809.094947E-1218.8232385
CTTCGTA1801.0913936E-1118.7397947
ACTAGAC3650.016.6338319
TCGTTGG1401.2038854E-616.1342034
TCTTCGT2151.4370016E-1015.7589916
GGGCGCG950.0052374514.2660343
CGTTGGA1656.6322864E-613.6896285
CCGCCAC1352.5647733E-413.3854144
TATCTTC7350.012.9073644
CGCCACC1952.6384387E-612.7418845
CGCCCGC1250.002185831512.6492171
CGGGGTT1250.002260977912.59242545
CGTGAGC1651.0050855E-412.3206641
TTCGTTG1902.8302573E-511.8883623
ATATCTT7700.011.7339683
CGCGGTG1400.00510399611.2939426
CTAGACA6000.010.86846320
Produced by FastQC (version 0.11.5)