FastQCFastQC Report
Mon 6 Jun 2016
ERR1391199.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391199.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743028
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA28120.16132844681783653No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA19790.11353804987642195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT3450.041.0859115
GCCGATG3600.039.9989816
CGTCATA3800.038.48586324
GCGGTCT3000.038.25012236
GGCGGTC3000.038.24902335
TAAGCCG3800.037.3016813
CCGATGA3950.037.02437217
GAATTCG3200.036.5615719
ATGACGT4050.036.1101920
AGGCGGT3200.035.8584634
CGGTATT3200.035.8584624
AATTCGG3300.035.4536420
ACGTCAT4250.034.94028523
GATGACG4200.034.8205419
AAGCCGA4100.034.57229214
TCGGTAT3350.034.25285723
TGACGTC4350.034.13706221
TTCGGTA3400.033.74913822
CGATGAC4300.033.4875218
CGGGGAA3450.033.2600215
Produced by FastQC (version 0.11.5)