FastQCFastQC Report
Mon 6 Jun 2016
ERR1391198.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391198.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400271
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA26530.1894633253134572No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA17930.12804664239993543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA3150.042.14192224
TAAGCCG3300.040.90818413
AGCCGAT3500.039.21341315
GCCGATG3450.039.12956616
CGGTATT2300.038.15132524
TGACGTC3500.037.92772721
GACGTCA3600.037.4991722
CCGATGA3550.037.3935317
CGATGAC3600.036.8741818
ATGACGT3700.035.87758320
TCGGTAT2450.035.8155323
GCGGTCT2500.035.09922436
GGCGGTC2500.035.09922435
GATGACG3800.034.93343719
AAGCCGA3900.034.61461314
ACGTCAT3850.034.47975523
AGGCGGT2550.034.41100334
TTCGGTA2550.034.41100322
TGCCGTC1250.032.40043645
AATTCGG2750.031.90838220
Produced by FastQC (version 0.11.5)