FastQCFastQC Report
Mon 6 Jun 2016
ERR1391197.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391197.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024577
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA17060.16650773929143442No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA12510.12209916873011985No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT11760.11477907468155152Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGTC2200.040.90855821
TAAGCCG2350.039.2548113
ATGACGT2300.039.12992520
CGATGAC2350.038.29737518
GGCGGTC2000.038.249535
ACGTCAT2400.037.4995123
CGTCATA2400.037.4995124
GCGGTCT2050.037.31658636
GATGACG2450.036.73421519
TATGCCG1350.036.66618743
TCGGTAT2100.036.42809723
TTCGGTA2200.035.79498722
CGGTATT2150.035.58093324
AGCCGAT2600.035.48030515
AATTCGG2200.034.77227420
CGGTCTC2200.034.77227437
GACGTCA2600.034.61493322
AAGCCGA2700.034.1662214
CCGATGA2650.033.96182617
TCGGGGA2300.033.26043714
Produced by FastQC (version 0.11.5)