FastQCFastQC Report
Mon 6 Jun 2016
ERR1391194.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391194.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137479
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA18280.16070626358816295No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA13120.11534278874599004No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT12330.10839760558216899Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT2600.039.8066815
CCGATGA2650.039.0556117
TATGCCG1350.038.33404543
GCCGATG2700.038.3323616
CGTCATA2700.038.3323624
CGATGAC2750.037.63540618
TAAGCCG2750.037.63540613
GTCGGAA301.1400848E-437.49904627
ATGACGT2800.036.96334520
GATGACG2950.035.08385519
TGACGTC3000.034.49912321
GATCGGA2100.034.28484326
GACGTCA3050.033.93356322
TCGGAAG2400.033.7491428
AAGCCGA3100.033.3862514
ACGTCAT3150.032.85630823
AGATCGG2150.032.44103625
TTCGGTA1950.032.3068722
CGGTATT2050.031.8284624
TGCCGTC1700.031.76529745
Produced by FastQC (version 0.11.5)