FastQCFastQC Report
Mon 6 Jun 2016
ERR1391193.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391193.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176893
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA28910.24564680051627463No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA21160.17979544444567178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG3500.041.78488516
GACGTCA3500.041.1420422
TAAGCCG3500.041.1420413
TGACGTC3550.040.56257621
AGCCGAT3650.039.4512715
CCGATGA3650.039.4512717
ATGACGT3700.038.91814820
GATGACG3700.038.91814819
AAGCCGA3800.037.89398614
CGTCATA3800.037.89398624
CGGTATT2950.037.37214324
TCGGTAT2950.037.37214323
GGCGGTC3000.036.7492735
ACGTCAT4000.036.56177523
AGATCGG1050.036.4278525
ATTCGGT3050.036.14682421
TCCTCGG2700.035.8326211
TTCCTCG2700.035.8326210
GCGGTCT3100.035.56381236
CGATGAC4100.035.12125418
Produced by FastQC (version 0.11.5)