FastQCFastQC Report
Mon 6 Jun 2016
ERR1391191.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391191.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446375
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA24560.5502100252030243No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA19130.42856342761131333No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT9600.21506580789694765Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTATGAAATCTCGTATGCCGTC6830.1530103612433492Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTATGAAATCTCGTATGCC6170.13822458695043405Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG551.8189894E-1244.99921443
TCGGGGA2400.042.18676814
AGCCGAT2500.041.39927715
GCCGATG2500.041.39927716
TAAGCCG2500.041.39927713
CGGGGAA2450.041.32581315
ATGCCGT603.6379788E-1241.24928344
TCCTCGG2250.040.99928711
CCTCGGG2250.040.99928712
TTCCTCG2250.040.99928710
CCGATGA2550.040.5875317
TGACGTC2550.040.5875321
GCGGTCT2500.040.49929436
TTCGGTA2500.040.49929422
AGATCGG950.040.26245525
CTCGGGG2300.040.10799813
ACGTCAT2600.039.80699523
CGATGAC2600.039.80699518
ATTCGGT2550.039.7051921
GGCGGTC2550.039.7051935
Produced by FastQC (version 0.11.5)