FastQCFastQC Report
Mon 6 Jun 2016
ERR1391189.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391189.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024729
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA47960.4680261805804266No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA32820.3202798008058716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG4900.042.2445816
AGCCGAT5000.041.8496815
TGACGTC5250.041.5711121
CGTCATA5300.040.7544124
CGATGAC5200.040.67276818
ATGACGT5400.040.4163620
CCGATGA5250.040.28540817
TAAGCCG5250.039.8568413
GATGACG5500.038.86334219
ACGTCAT5600.038.57113623
AAGCCGA5500.038.04516614
GGCGGTC4150.037.95152335
TCGGTAT4150.037.95152323
GACGTCA5750.037.5649322
AGATCGG1200.037.49971825
TTCCTCG3600.037.49971410
GCGGTCT4350.036.2066236
ATTCGGT4350.036.2066221
AGGCGGT4350.036.2066234
TTCGGTA4350.036.2066222
Produced by FastQC (version 0.11.5)