FastQCFastQC Report
Mon 6 Jun 2016
ERR1391188.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391188.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710374
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA27120.3817707292215087No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA17580.24747527358827884No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT14730.20735556199973532Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATTAAGATCTCGTATGCCGTC9600.13514008114035705Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTCG700.041.7850610
GCCGATG2750.041.72662416
TAAGCCG2750.041.72662413
AGCCGAT2800.040.98150315
CGTCATA2800.040.17794424
TCGGTAT3050.039.8354423
TGACGTC2850.039.4730721
GGCGGTC3200.039.37438235
ATTCGGT3100.039.19293221
CCGATGA2900.038.79249617
ACGTCAT2900.038.79249623
GCGGTCT3250.038.76862736
TTCGGTA3150.038.57082422
CGGTATT3150.038.57082424
GATGACG2950.038.13519
CGATGAC2950.038.13518
AAGCCGA3050.037.62236414
GACGTCA3000.037.49941622
ATGACGT3000.037.49941620
TCCTCGG2700.037.49941311
Produced by FastQC (version 0.11.5)