FastQCFastQC Report
Mon 6 Jun 2016
ERR1391186.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391186.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862198
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA31690.3675489852678851No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA21660.25121839763024273No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT9400.10902368133537772Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGAC3300.040.9079818
TTCGGTA2450.040.40706622
AGCCGAT3400.040.36655415
TAAGCCG3400.039.70480713
CCGATGA3500.039.21322317
GCCGATG3500.039.21322316
CGGTATT2600.038.94125424
ATTCGGT2550.038.8224821
GCGGTCT2500.038.6989536
GGCGGTC2500.038.6989535
TCGGTAT2600.038.07589323
GACGTCA3650.037.60172322
TGACGTC3650.037.60172321
GATGACG3650.036.985319
ATGACGT3750.036.5990120
AAGCCGA3850.036.23278414
CAGGCGG2750.035.99902333
CTCGGGG1900.035.52535613
CGTCATA3800.035.52535624
ACGTCAT3850.035.06398423
Produced by FastQC (version 0.11.5)