FastQCFastQC Report
Mon 6 Jun 2016
ERR1391185.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391185.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122865
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA27770.24731379106125848No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA19880.17704710717673094No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT12170.10838346551010139Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG2900.040.3441913
GCCGATG3050.039.09774416
CCGATGA3200.037.96814717
AGCCGAT3100.037.7413415
CGTCATA3150.037.1422724
CGATGAC3200.036.5619218
GCGGTCT2600.036.34557736
GACGTCA3250.035.9994322
AAGCCGA3250.035.9994314
GGCGGTC2650.035.6598135
AATTCGG2650.035.6598120
ATGACGT3300.035.45398720
ACGTCAT3300.035.45398723
GATGACG3300.035.45398719
TCGGTAT2700.034.99944723
GAATTCG2750.034.36309419
CGGGGAA2850.033.9468315
TGACGTC3450.033.91250621
ATTCGGT2950.032.03339421
CGGTATT2950.032.03339424
Produced by FastQC (version 0.11.5)