FastQCFastQC Report
Mon 6 Jun 2016
ERR1391184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035016
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA35420.3422169319121637No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA23750.2294650517479923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTC2950.041.94757535
TTCGGTA2900.041.8949822
GCGGTCT3000.041.25044636
CGATGAC4150.041.2032718
GATGACG4200.040.71275319
TCGGTAT3000.040.49847823
AGGCGGT2950.040.42220734
TAAGCCG4250.040.23378413
TCGGGGA3050.039.83456814
AATTCGG3050.039.83456820
AGCCGAT4300.039.7659515
GACGTCA4300.039.7659522
GCCGATG4350.039.3088716
ATGACGT4350.039.3088720
CCGATGA4400.038.8621817
CGTCATA4400.038.8621824
TGACGTC4450.038.42551821
CGGTATT3200.037.96732324
GAATTCG3200.037.96732319
CAGGCGG3200.037.26422533
Produced by FastQC (version 0.11.5)