FastQCFastQC Report
Mon 6 Jun 2016
ERR1391182.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391182.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034151
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA41110.39752415266242547No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA27480.26572521807743743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGACG4950.042.7257119
TAAGCCG5000.041.84847313
GCCGATG4950.041.81665416
CGATGAC5050.041.4341318
CGTCATA5050.041.4341324
AGCCGAT5000.041.39848715
GACGTCA5100.041.02791622
ATGACGT5100.041.02791620
CCGATGA5050.040.988617
TGACGTC5200.040.23891421
ACGTCAT5350.039.95181323
CGGTATT4650.038.2244124
ATTCGGT4600.038.1507821
TCGGTAT4600.038.1507823
GGCGGTC4500.037.99861535
AATTCGG4700.037.8177720
AAGCCGA5600.037.36470814
TTCGGTA4700.037.3390622
GAATTCG4850.036.64814419
CGGGGAA4850.036.18424215
Produced by FastQC (version 0.11.5)