FastQCFastQC Report
Mon 6 Jun 2016
ERR1391181.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391181.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717364
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA55500.3231696949510995No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA35960.20939067081876644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT6250.042.4789315
CCGATGA6250.042.4789317
GCCGATG6350.041.80996716
TAAGCCG6400.041.48332613
GACGTCA6550.041.22033322
CGTCATA6500.040.84512324
CGATGAC6650.039.92380518
AAGCCGA6750.039.33234414
GATGACG6750.039.33234419
TGACGTC6900.039.12944821
ACGTCAT6950.038.20047423
ATGACGT7050.037.65862720
GGCGGTC5100.037.49905435
TCGGTAT5200.036.7779223
GCGGTCT5300.036.08399236
TTCGGTA5300.036.08399222
TCGGGGA5350.035.7467614
CGGTATT5350.035.7467624
ATTCGGT5400.035.4157721
AGGCGGT5500.034.7718534
Produced by FastQC (version 0.11.5)