FastQCFastQC Report
Mon 6 Jun 2016
ERR1391180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340994
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA40090.29895734059958506No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA26780.19970260866193287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG3850.042.66166713
GCCGATG4000.041.62434816
TGACGTC4000.040.49936321
CCGATGA4200.040.1779417
ATGACGT4200.039.1065320
AGCCGAT4300.038.72031815
AAGCCGA4300.038.72031814
GACGTCA4200.038.57082422
CGTCATA4200.038.57082424
TCGGTAT4500.037.999423
TTCCTCG3750.037.79940410
CGATGAC4350.037.7580318
GCGGTCT4550.037.5818336
ATTCGGT4550.037.5818321
ACGTCAT4350.037.24079523
TCGGGGA4600.037.17332514
GGCGGTC4600.037.17332535
TCCTCGG3900.036.92249711
GATTCGG800.036.56192811
GATGACG4500.036.49942419
Produced by FastQC (version 0.11.5)