FastQCFastQC Report
Mon 6 Jun 2016
ERR1391178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1553578
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA42100.2709873595017437No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA29160.18769575779265668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG5500.040.09033613
CCGATGA5550.039.3237617
CGTCATA5600.038.97265624
ATGACGT5700.038.68365520
GATGACG5750.037.9559819
TGACGTC5800.037.62877321
CGATGAC5800.037.62877318
GCCGATG5850.037.3071616
GGCGGTC4050.037.2216935
AGCCGAT5900.036.99099315
GACGTCA5900.036.99099322
TATGCCG1100.036.81765743
AATTCGG4150.036.3247820
TCGGTAT4200.035.89234523
ACGTCAT6200.035.56400723
TTCGGTA4350.035.17191322
GCGGTCT4400.034.77223236
AAGCCGA6350.034.72391514
CGGTATT4350.034.6546824
GAATTCG4500.033.99951619
Produced by FastQC (version 0.11.5)