FastQCFastQC Report
Mon 6 Jun 2016
ERR1391176.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391176.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910670
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA30580.33579672109545716No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA21270.23356429881296187No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT12400.13616348402824294Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCTGAATCTCGTATGCCGTC9580.10519727233794897Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG4000.042.7490113
TTCGGTA2950.040.4227922
TCGGTAT2950.040.4227923
GCGGTCT2900.040.3438936
CCGATGA4300.039.7665217
GGCGGTC3000.039.74907735
CGGTATT3000.039.74907724
ATGACGT4250.039.7049620
AGCCGAT4300.039.2432815
GACGTCA4300.039.2432822
TGACGTC4300.039.2432821
CGGGGAA3050.039.09745815
AATTCGG3050.039.09745820
GCCGATG4350.038.79220216
GATGACG4450.038.42607519
CGATGAC4450.038.42607518
CGGTCTC3050.038.35976837
TATGCCG1350.038.3345543
AAGCCGA4500.037.99912314
GAATTCG3150.037.85626219
Produced by FastQC (version 0.11.5)