FastQCFastQC Report
Mon 6 Jun 2016
ERR1391175.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391175.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394736
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA32220.2310114602333345No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA21640.1551548106595083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG4400.040.3970216
CGATGAC4450.039.9431218
GACGTCA4400.039.88566222
TAAGCCG4500.039.4993113
AGCCGAT4550.039.06524715
TGACGTC4550.039.06524721
GATGACG4700.037.81848519
AAGCCGA4850.037.57666414
CGTCATA4700.037.33976724
CCGATGA4850.036.64884617
ACGTCAT4800.036.5618623
CGGTATT3500.035.99936724
GGCGGTC3450.035.86893535
TCCTCGG3100.034.838111
TGCCGTC1750.034.71367645
TCGGTAT3650.034.51994323
GCGGTCT3600.034.37439736
TCGGGGA3750.034.199414
ATGACGT5250.033.8565520
TTCCTCG3200.033.7494110
Produced by FastQC (version 0.11.5)