FastQCFastQC Report
Mon 6 Jun 2016
ERR1391174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155611
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA39790.3443200177222266No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA27640.2391808316120217No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT13140.11370608275622161Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT4150.042.2875315
TAAGCCG4200.041.78410313
CGATGAC4250.041.2925318
GCGGTCT3600.041.250236
GCCGATG4300.040.81238616
CCGATGA4350.040.34327717
TGACGTC4400.039.88655521
CGTCATA4450.039.43839324
AGGCGGT3800.039.07744234
GGCGGTC3800.039.07744235
GACGTCA4500.039.00018722
AAGCCGA4550.038.56994614
GATGACG4550.038.56994619
TCGGGGA3900.038.07545514
ATGACGT4700.037.81933220
AATTCGG4000.037.68768320
CGGTCTC4000.037.1251837
ACGTCAT4750.036.94754823
TCCTCGG3300.036.8167711
CGGTATT4100.036.76846724
Produced by FastQC (version 0.11.5)