FastQCFastQC Report
Mon 6 Jun 2016
ERR1391172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519183
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA30810.593432373556145No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA19850.3823314707916091No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT11820.2276653896602932Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTAGAATCTCGTATGCCGTC9060.1745049433436765Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTAGAATCTCGTATGCC5400.10400956888033698Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGTC3300.043.6357121
GCCGATG3350.042.98443216
TAAGCCG3350.042.98443213
AGCCGAT3450.041.73850615
ATGACGT3450.041.73850620
CGTCATA3450.041.73850624
GACGTCA3500.041.14224222
CGATGAC3500.041.14224218
TCGGGGA2800.040.9815314
GATTCGG556.002665E-1140.9084811
GATGACG3550.040.5627719
AATTCGG2850.040.26255420
TTCCTCG2350.040.21216610
CCGATGA3600.039.999417
AAGCCGA3600.039.999414
TTCGGTA2900.039.56837522
TCGGTAT2900.039.56837523
TATGCCG1200.039.37441343
ATGCCGT1150.039.1298544
ATTCGGT2950.038.8977221
Produced by FastQC (version 0.11.5)