FastQCFastQC Report
Mon 6 Jun 2016
ERR1391171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739386
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA28390.3839672376809948No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA18200.24615018407164865No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT10740.1452556580730498Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAGCATCTCGTATGCCGTC7700.10414046249185135Illumina Paired End PCR Primer 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCT2800.043.39207536
CGATGAC3050.042.0484218
TAAGCCG3050.042.0484213
AGGCGGT2900.041.89579434
CGGTATT2850.041.8413524
TCGGTAT2850.041.8413523
GCCGATG3100.041.37022416
CGTCATA3100.041.37022424
GGCGGTC2950.041.18569635
TTCGGTA2900.041.11994622
CCGATGA3150.040.7135517
GACGTCA3150.040.7135522
AATTCGG2950.040.42299720
TGACGTC3200.040.077421
ACGTCAT3200.040.077423
GATGACG3200.040.077419
CAGGCGG3100.039.91863633
GATCGGA1300.039.80697626
TCGGGGA3000.039.74928314
ATTCGGT3000.039.74928321
Produced by FastQC (version 0.11.5)