FastQCFastQC Report
Mon 6 Jun 2016
ERR1391170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030364
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA41970.40733177789596686No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA29130.2827156228284373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG4700.043.56284713
CGTCATA4850.042.21553824
CCGATGA4900.041.78476717
CGATGAC4900.041.78476718
AAGCCGA5000.041.3990614
GATGACG4950.041.36269819
TGACGTC5050.040.9891721
AGCCGAT5100.040.5873215
ATGACGT5050.040.54363320
ACGTCAT5050.040.54363323
TCCTCGG3600.039.99909211
CCTCGGG3550.039.9286712
CTCGGGG3550.039.9286713
GCGGTCT4400.039.8854636
GCCGATG5150.039.75638216
GGCGGTC4550.039.55954435
TCGGGGA4550.039.0650514
AATTCGG4650.038.70880520
TTCGGTA4650.038.70880522
GAATTCG4650.038.70880519
Produced by FastQC (version 0.11.5)