FastQCFastQC Report
Mon 6 Jun 2016
ERR1391168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182126
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA39220.3317751238023696No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA27090.22916338867430375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG5050.041.8803213
TGACGTC5100.041.4697321
AGCCGAT5050.041.43478815
GCCGATG5000.041.3991416
CCGATGA5050.040.9892517
CGATGAC5050.040.9892518
GACGTCA5200.040.67223722
GATGACG5200.039.8068719
CGTCATA5200.039.8068724
AAGCCGA5350.039.53189514
ACGTCAT5400.039.1658623
ATGACGT5300.039.05579820
GGCGGTC4150.038.4931835
GCGGTCT4300.038.19688436
TCGGTAT4200.038.03492723
TTCGGTA4350.037.2406122
AGGCGGT4350.036.72337734
CGGTCTC4500.036.49924537
CGAAGTA250.002107346735.99925639
TCGGGGA4500.035.99925214
Produced by FastQC (version 0.11.5)