FastQCFastQC Report
Mon 6 Jun 2016
ERR1391167.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391167.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227529
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA13150.5779483054907286No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA8670.38105032765054125No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT3340.14679447455049685Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCAGAATCTCGTATGCCGTC2380.10460205072759957Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT1900.041.44655615
ATGACGT1900.041.44655620
GATGACG1900.041.44655619
TAAGCCG1900.041.44655613
CTCGGGG1050.040.7134913
GCCGATG1950.040.38382716
AAGCCGA1950.040.38382714
ATCGGAA451.917033E-839.99921827
TCGGGGA1300.039.80691514
GGCGGTC1300.039.80691535
GACGTCA2000.039.3742322
CGATGAC2000.039.3742318
ATGCCGA800.039.3742319
TCCTCGG1100.038.86287711
TTCCTCG1100.038.86287710
CCGATGA2050.038.41388317
TGACGTC2050.038.41388321
CGTCATA2050.038.41388324
CGGGGAA1350.038.33258415
CGGTCTC1350.038.33258437
Produced by FastQC (version 0.11.5)